Anti-GNL3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9BVP2
Gene Id	26354
Gene Symbols	GNL3
Protein Name / Synonyms	GNL3, C77032, E2IG3, NNP47, NS, G protein nucleolar 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100
IP 1:50 - 1:100

Format

Calculated MW

60kDa/61kDa

Observed MW

62kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of 293T cells, using GNL3 antibody (144-06459) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - GNL3 Polyclonal Antibody (144-06459)

Immunofluorescence analysis of MCF7 cells using GNL3 antibody (144-06459). Blue: DAPI for nuclear staining.
Immunofluorescence - GNL3 Polyclonal Antibody (144-06459)

Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug GNL3 antibody (144-06459). Western blot was performed from the immunoprecipitate using GNL3 antibody (144-06459) at a dilition of 1:500.
Immunoprecipitation - GNL3 Polyclonal Antibody (144-06459)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products