RayBiotech

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Anti-GNRH1 Antibody

Catalog #: 144-05625

GNRH1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P01148
Gene Id
2796
Gene Symbols
GNRH1
Protein Name / Synonyms
GNRH1, GNRH, GRH, LHRH, LNRH, progonadoliberin-1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
10kDa
Observed MW
12kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 24-92 of human GNRH1 (NP_001076580.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GNRH1 antibody (144-05625) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - GNRH1 antibody (144-05625)

Immunohistochemistry of paraffin-embedded human prostate using GNRH1 antibody (144-05625) at dilution of 1:100 (40x lens).
Immunohistochemistry - GNRH1 antibody (144-05625)

Immunohistochemistry of paraffin-embedded human stomach using GNRH1 antibody (144-05625) at dilution of 1:100 (40x lens).
Immunohistochemistry - GNRH1 antibody (144-05625)

Expiration:
12 months from the date of shipment when stored properly.