Anti-GOLGA1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q92805
Gene Id	2800
Gene Symbols	GOLGA1
Protein Name / Synonyms	GOLGA1, golgin-97, golgin A1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein is associated with Sjogren's syndrome.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

88kDa

Observed MW

105kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA1 (NP_002068.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GOLGA1 antibody (144-60750) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - GOLGA1 antibody (144-60750)

Immunofluorescence analysis of HepG2 cells using GOLGA1 antibody (144-60750) at dilution of 1:150. Blue: DAPI for nuclear staining.
Immunofluorescence - GOLGA1 antibody (144-60750)

Expiration:

12 months from the date of shipment when stored properly.

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