Anti-GOLGA2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q08379
Gene Id	2801
Gene Symbols	GOLGA2
Protein Name / Synonyms	GOLGA2, GM130, golgin A2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Concentration (lot specific)	2.96 mg/ml
Buffers	PBS with 0.05% proclin300, 50% glycerol, pH 7.3
Recommended Applications	Western Blotting, IF, IP
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

70kDa/113kDa

Observed MW

145kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GOLGA2 (NP_004477.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GOLGA2 antibody (144-05344) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - GOLGA2 Polyclonal Antibody (144-05344)

Immunofluorescence analysis of HeLa cells using GOLGA2 antibody (144-05344) at dilution of 1:400 (60x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - GOLGA2 Polyclonal Antibody (144-05344)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug GOLGA2 antibody (144-05344). Western blot was performed from the immunoprecipitate using GOLGA2 antibody (144-05344) at a dilition of 1:1000.
Immunoprecipitation - GOLGA2 Polyclonal Antibody (144-05344)

Expiration:

12 months from the date of shipment when stored properly.