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Anti-GOLGA2 Antibody
Catalog #: 144-05344
GOLGA2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q08379 |
| Gene Id | 2801 |
| Gene Symbols | GOLGA2 |
| Protein Name / Synonyms | GOLGA2, GM130, golgin A2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Concentration (lot specific) | 2.96 mg/ml |
| Buffers | PBS with 0.05% proclin300, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
70kDa/113kDa
Observed MW
145kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GOLGA2 (NP_004477.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GOLGA2 antibody (144-05344) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using GOLGA2 antibody (144-05344) at dilution of 1:400 (60x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug GOLGA2 antibody (144-05344). Western blot was performed from the immunoprecipitate using GOLGA2 antibody (144-05344) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.