RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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ISO 13485:2016 cGMP

Anti-GOLGA4 Antibody

Catalog #: 144-10216

GOLGA4 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q13439
Gene Id
2803
Gene Symbols
GOLGA4
Protein Name / Synonyms
GOLGA4, CRPF46, GCP2, GOLG, MU-RMS-40.18, p230, golgin A4
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Shipping TypeBlue ice
Storage-20°C

Description

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein has been postulated to play a role in Rab6-regulated membrane-tethering events in the Golgi apparatus. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Dilutions

WB 1:1000 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
260kDa/261kDa/262kDa
Observed MW
280kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GOLGA4 (NP_002069.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GOLGA4 antibody (144-010216) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - GOLGA4 Polyclonal Antibody (144-010216)

Expiration:
12 months from the date of shipment when stored properly.