Anti-GOLGA5 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8TBA6
Gene Id	9950
Gene Symbols	GOLGA5
Protein Name / Synonyms	GOLGA5, GOLIM5, RFG5, ret-II, golgin A5
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

78kDa/83kDa

Observed MW

100kDa, 115kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GOLGA5 (NP_005104.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GOLGA5 antibody (144-61189) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - GOLGA5 antibody (144-61189)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products