RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-Golgi Antibody, Sodium Azide Free

Catalog #: 188-10887-SAF

Mouse anti-Human Golgi Antibody [Sodium Azide Free]

Product Description

Specifications

Size100 µg
Estimated Lead Time1-2 weeks
SpeciesHuman
Specificity
Does not react with mouse and rat. Other species not tested.
Host SpeciesMouse
Protein Name / Synonyms
Golgi
Immunogen
SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.
IsotypeKappa, IgG1
Purity
Protein G affinity chromatography
Concentration (lot specific)
1 mg/ml in 1X PBS, BSA free, sodium azide free
Recommended Applications
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence: 0.5-1ug/ml
Western blot: 0.5-1ug/ml
Immunocytochemistry (Acetone or paraformaldehyde fixed): 0.5-1ug/ml for 30 min
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
Domain / Region / TerminusGolgi complex in cytoplasm
Shipping TypeBlue ice
Storage≤-20°C

Description

Description

This mAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

Storage

Store the Golgi antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.