RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-GOPC Antibody

Catalog #: 144-07513

GOPC Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9HD26
Gene Id
57120
Gene Symbols
GOPC
Protein Name / Synonyms
GOPC, CAL, FIG, GOPC1, PIST, dJ94G16.2, golgi associated PDZ and coiled-coil motif containing
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
35kDa/49kDa/50kDa
Observed MW
51kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 195-454 of human GOPC (NP_001017408.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GOPC antibody (144-07513) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - GOPC Polyclonal Antibody (144-07513)

Immunofluorescence analysis of A549 cells using GOPC antibody (144-07513). Blue: DAPI for nuclear staining.
Immunofluorescence - GOPC Polyclonal Antibody (144-07513)

Expiration:
12 months from the date of shipment when stored properly.