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ISO 13485:2016 cGMP
Anti-GOT1 Antibody
Catalog #: 144-05822
GOT1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P17174 |
| Gene Id | 2805 |
| Gene Symbols | GOT1 |
| Protein Name / Synonyms | GOT1, AST1, ASTQTL1, GIG18, cAspAT, cCAT, glutamic-oxaloacetic transaminase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100
IHC 1:50 - 1:200
IF 1:10 - 1:100
Format
Calculated MW
44kDa/46kDa
Observed MW
42kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 244-413 of human GOT1 (NP_002070.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GOT1 antibody (144-05822) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat heart using GOT1 antibody (144-05822) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using GOT1 antibody (144-05822) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using GOT1 antibody (144-05822) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GOT1 antibody (144-05822). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.