Anti-GOT2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P00505
Gene Id	2806
Gene Symbols	GOT2
Protein Name / Synonyms	GOT2, KAT4, KATIV, KYAT4, mitAAT, glutamic-oxaloacetic transaminase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

43kDa/47kDa

Observed MW

48kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human GOT2 (NP_002071.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GOT2 antibody (144-06915) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - GOT2 Polyclonal Antibody (144-06915)

Western blot analysis of extracts of various cells, using GOT2 antibody (144-06915) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - GOT2 Polyclonal Antibody (144-06915)

Expiration:

12 months from the date of shipment when stored properly.

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