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Anti-GPI (C-term) Antibody

Catalog #: 102-18393

Rabbit Anti-GPI (C-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P06744
Gene Id
2821
Gene Symbols
GPI
Protein Name / Synonyms
GPI (C-term)
ClonalityPolyclonal
PreparationThis GPI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human GPI.
Concentration (lot specific)
0.5 mg/ml
Recommended ApplicationsWestern Blotting, FC
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

GPI belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Specificity

Predicted Reactivity
Monkey
Other Names
GPI; Glucose-6-phosphate isomerase; Autocrine motility factor; Neuroleukin; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36
NCBI Accession #
NP_000166.2;NP_001171651.1
Other Accession #
Q4R591

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
63147
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
Western blot analysis of lysates from Ramos cell line,mouse liver,rat liver tissue (from left to right),using GPI Antibody (C-term)(Cat# 102-18393).102-18393 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
GPI Antibody (C-term) (Cat# 102-18393) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.