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Anti-GPLD1 Antibody
Catalog #: 144-06612
GPLD1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P80108 |
| Gene Id | 2822 |
| Gene Symbols | GPLD1 |
| Protein Name / Synonyms | GPLD1, GPIPLD, GPIPLDM, PIGPLD, PIGPLD1, PLD, phosphatidylinositol-glycan-specific phospholipase D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.05% proclin300, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:100
Format
Calculated MW
19kDa/92kDa
Observed MW
90kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 24-160 of human GPLD1 (NP_001494.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GPLD1 antibody (144-06612) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat heart using GPLD1 antibody (144-06612) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using GPLD1 antibody (144-06612) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using GPLD1 antibody (144-06612) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using GPLD1 antibody (144-06612). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.