Anti-GPX1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P07203
Gene Id	2876
Gene Symbols	GPX1
Protein Name / Synonyms	GPX1 (C-term)
Clonality	Polyclonal
Preparation	This GPX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human GPX1.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	FC, Western Blotting, IHC-P, IF
Research Area	Angiogenesis, Cardiovascular Disease, Inflammation, Oxidative Stress
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

GPX1 encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence.

Specificity

Other Names

GPX1; Glutathione peroxidase 1; Cellular glutathione peroxidase

NCBI Accession #

NP_000572.2;NP_958799.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22088

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of lysates from THP-1 cell line ?mouse liver and rat liver tissue (from left to right),using GPX1 Antibody (C-term)(Cat# 102-18227).102-18227 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with GPX1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of GPX1 Antibody (C-term)(Cat# 102-18227) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	GPX1 Antibody (C-term) (Cat# 102-18227) flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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