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Anti-GRB2 (Center) Antibody
Catalog #: 102-16724
Rabbit Anti-Human GRB2 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P62993 |
| Gene Id | 2885 |
| Gene Symbols | GRB2 |
| Protein Name / Synonyms | GRB2 (Center) |
| Clonality | Polyclonal |
| Preparation | This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the Central region of human GRB2. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
GRB2 binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences.
Specificity
Predicted Reactivity
Mouse, Rat, Xenopus
Other Names
GRB2; ASH; Growth factor receptor-bound protein 2; Adapter protein GRB2; Protein Ash; SH2/SH3 adapter GRB2
NCBI Accession #
NP_002077.1;NP_987102.1
Other Accession #
P62994; Q60631; Q6GPJ9; P87379
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
25206
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100
 | Western blot analysis of GRB2 Antibody (Center) (Cat# 102-16724) in A2058 cell line lysates (35ug/lane). GRB2 (arrow) was detected using the purified Pab. |
 | Western blot analysis of GRB2 (arrow) using GRB2 Antibody (Center) (Cat# 102-16724). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.