RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-GSTK1 Antibody

Catalog #: 144-05226

GSTK1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
Q9Y2Q3
Gene Id
373156
Gene Symbols
GSTK1
Protein Name / Synonyms
GSTK1, GST, GST 13-13, GST13, GST13-13, GSTK1-1, hGSTK1, glutathione S-transferase kappa 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW
20kDa/24kDa/25kDa/31kDa
Observed MW
25kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GSTK1 antibody (144-05226) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - GSTK1 Polyclonal Antibody (144-05226)

Immunofluorescence analysis of Hela cells using GSTK1 antibody (144-05226) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - GSTK1 Polyclonal Antibody (144-05226)

Expiration:
12 months from the date of shipment when stored properly.