Anti-GTF2H3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q13889
Gene Id	2967
Gene Symbols	GTF2H3
Protein Name / Synonyms	GTF2H3, BTF2, P34, TFB4, TFIIH, general transcription factor IIH subunit 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the TFB4 family. The encoded protein is a subunit of the core-TFIIH basal transcription factor and localizes to the nucleus. The encoded protein is involved in RNA transcription by RNA polymerase II and nucleotide excision repair and associates with the Cdk-activating kinase complex. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 14.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW

29kDa/34kDa

Observed MW

36kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-308 of human GTF2H3 (NP_001507.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using GTF2H3 antibody (144-07188) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - GTF2H3 Polyclonal Antibody (144-07188)

Immunofluorescence analysis of A549 cells using GTF2H3 antibody (144-07188). Blue: DAPI for nuclear staining.
Immunofluorescence - GTF2H3 Polyclonal Antibody (144-07188)

Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug GTF2H3 antibody (144-07188). Western blot was performed from the immunoprecipitate using GTF2H3 antibody (144-07188) at a dilition of 1:1000.
Immunoprecipitation - GTF2H3 Polyclonal Antibody (144-07188)

Expiration:

12 months from the date of shipment when stored properly.

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