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Anti-GTF2I Antibody
Catalog #: 144-07898
GTF2I Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P78347 |
| Gene Id | 2969 |
| Gene Symbols | GTF2I |
| Protein Name / Synonyms | GTF2I, BAP135, BTKAP1, DIWS, GTFII-I, IB291, SPIN, TFII-I, WBS, WBSCR6, general transcription factor IIi |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a phosphoprotein containing six characteristic repeat motifs. The encoded protein binds to the initiator element (Inr) and E-box element in promoters and functions as a regulator of transcription. This locus, along with several other neighboring genes, is deleted in Williams-Beuren syndrome. There are many closely related genes and pseudogenes for this gene on chromosome 7. This gene also has pseudogenes on chromosomes 9, 13, and 21. Alternatively spliced transcript variants encoding multiple isoforms have been observed.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200
IHC 1:100 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
30kDa/107kDa/110kDa/112kDa
Observed MW
132kDa
Immunogen
A synthetic peptide of human GTF2I
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of BT-474 cells, using GTF2I antibody (144-07898) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot analysis of extracts of mouse brain, using GTF2I antibody (144-07898) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using GTF2I antibody (144-07898) (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using GTF2I antibody (144-07898) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using GTF2I antibody (144-07898) (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using GTF2I antibody (144-07898) (40x lens).
Immunofluorescence analysis of A431 cells using GTF2I antibody (144-07898) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.