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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q02108 |
| Gene Id | 2982 |
| Gene Symbols | GUCY1A3 |
| Protein Name / Synonyms | GUCY1A3, GC-SA3, GUC1A3, GUCA3, GUCSA3, GUCY1A1, MYMY6, guanylate cyclase soluble subunit alpha-3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.
Dilutions
WB 1:1000 - 1:3000
Format
Calculated MW
70kDa/77kDa
Observed MW
77kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human GUCY1A3 (NP_000847.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GUCY1A3 antibody (144-12710) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.