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ISO 13485:2016 cGMP
Anti-GUCY1B3 Antibody
Catalog #: 144-60971
GUCY1B3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q02153 |
| Gene Id | 2983 |
| Gene Symbols | GUCY1B3 |
| Protein Name / Synonyms | GUCY1B3, GC-S-beta-1, GC-SB3, GUC1B3, GUCB3, GUCSB3, GUCY1B1, guanylate cyclase soluble subunit beta-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:200 - 1:2000
Format
Calculated MW
66kDa/68kDa/70kDa
Observed MW
70kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human GUCY1B3 (NP_000848.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using GUCY1B3 antibody (144-015276) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30S.
Expiration:
12 months from the date of shipment when stored properly.