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Anti-H2AFZ Antibody
Catalog #: 144-06614
H2AFZ Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P0C0S5 |
| Gene Id | 3015 |
| Gene Symbols | H2AFZ |
| Protein Name / Synonyms | H2AFZ, H2A.Z-1, H2A.z, H2A/z, H2AZ, histone H2A.Z |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
13kDa
Observed MW
15kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-128 of human H2AFZ (NP_002097.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using H2AFZ antibody (144-06614) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse kidney using H2AFZ antibody (144-06614) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using H2AFZ antibody (144-06614) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using H2AFZ antibody (144-06614) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.