Anti-H6PD Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O95479
Gene Id	9563
Gene Symbols	H6PD
Protein Name / Synonyms	H6PD, CORTRD1, G6PDH, GDH, GDH/6PGL endoplasmic bifunctional protein
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

There are 2 forms of glucose-6-phosphate dehydrogenase. G form is X-linked and H form, encoded by this gene, is autosomally linked. This H form shows activity with other hexose-6-phosphates, especially galactose-6-phosphate, whereas the G form is specific for glucose-6-phosphate. Both forms are present in most tissues, but H form is not found in red cells.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

88kDa

Observed MW

89kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human H6PD (NP_004276.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using H6PD Antibody (144-06440) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - H6PD Polyclonal Antibody (144-06440)

Immunohistochemistry of paraffin-embedded rat kidney using H6PD antibody (144-06440) at dilution of 1:200 (40x lens).
Immunohistochemistry - H6PD Polyclonal Antibody (144-06440)

Immunofluorescence analysis of U2OS cells using H6PD antibody (144-06440). Blue: DAPI for nuclear staining.
Immunofluorescence - H6PD Polyclonal Antibody (144-06440)

Expiration:

12 months from the date of shipment when stored properly.

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