Anti-HA Tag Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Host Species	Rabbit
Protein Name / Synonyms	HA Tag
Clonality	Polyclonal
Preparation	KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, CHIP
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYAYPYDVPDYA) is an established example. RayBiotech's anti-HA polyclonal antibody provides a simple solution to detect the expression of an HA-tagged protein in cells.

Specificity

Other Names

Glutathione S-transferase

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

25712 Da

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; CHIP: 1:100

	Western blot analysis of lysate from 12 tag recombinant protein cell line, using Tag-HA. 2x Antibody (Cat# 102-10559). 102-10559 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
	Western blot analysis of anti-HA tag pab(Cat# 102-10559)in HA-tagged recombinant protein bacterial lysate.
	GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))
	ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007))