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Anti-HA-Tag Antibody
Catalog #: RB-IP-10-101
Mouse Anti-HA-Tag Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Specificity | HA-Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot. |
| Protein Name / Synonyms | HA-Tag |
| Clonality | Monoclonal |
| Immunogen | Conjugated-Peptide with sequence YPYDVPDYA was used as immunogen. The antibody was purified from cell culture supernatantby affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 µg/ml) |
| Reconstitution | Lyophilized mouse anti-HA-Tag antibody is stable at 4°C and below -18°C. Supplied as 100 µg of purified antibody. Reconstitute with sterile water. If 1 ml of water is used, the antibody concentration will be 100 µg/ml. Should this product contain a powder we recommend microcentrifugation before use. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA and GFP. HA Tag is very common using epitope tags. The tags are encoded by a variety of expression vectors, and effective vectors suitable for the production of recombinant tagged fusion proteins. Each expression vector encodes a unique, nine amino acids HATag epitope sequence. Anti-HA-Tag antibodies are suitable for purification, detection and immunoprecipitation of recombinant fusion proteins containing the respective HA-Tag.
Typical Data
Storage/Stability
Lyophilized samples are stable for a year at least from date of receipt when stored at -20° C to -70° C. Upon reconstitution, the antibody can be stored at 2° - 8° C for at least a month without detectable loss of activity. Minimize freezing and thawing
Expiration
12 months from the date of shipment when stored properly.
References
- Yang RZ, et al..(2006) Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action. Am J Physiol Endocrinol Metab. 290(6):E1253-61.
- Schäffler A, et al.. (2005) Genomic structure of human omentin, a new adipocytokine expressed in omental adipose tissue. Biochim Biophys Acta. 1732(1-3):96-102.