RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-HACD2 (LD20) Antibody

Catalog #: 131-0169

Human HACD2 Antibody. Peptide: LD-20 (65-LAKGSYHSLYYSIEKPLKFC-83)

Product Description

Specifications

Size20 µg, 100 µg, 200 µg, 500 µg, 1 mg
Estimated Lead Time1-2 business days
SpeciesHuman
Host SpeciesRabbit
ClonalityPolyclonal
Immunogen
This antibody was produced from antiserum of rabbits immunized with a recombinant protein immunogen. The IgG fraction was purified by Protein A affinity chromatography.
Immunogen Sequence
LAKGSYHSLYYSIEKPLKFC
Recommended ApplicationsELISA (recommended work dilution = 1:5, 000)
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

The HACD2 (3-Hydroxyacyl-CoA Dehydratase 2) is a Protein Coding gene. Diseases associated with HACD2 include Unilateral Focal Polymicrogyria and Spinocerebellar Ataxia.The encoded protein localizes to the endoplasmic reticulum membrane.

Typical Data

IHC shows positive staining in paraffin-embedded tumor tissue. Antibody Rabbit Anti-Human HACD2 (LD-20) 131-0169 was used at a dilution of 1/2000 followed by mouse and rabbit IgG (ready to use) polymerization using HRP polymer, counterstained with hematoxylin. Heat-mediated antigen retrieval was performed using Tris/EDTA buffer pH9.0 before starting IHC staining (40X).

Immunofluorescence staining of rabbit Anti-human HACD2(LD-20) antibody in MCF-7 and HepG2 cells. Incubate with rabbit anti-human HACD2(LD-20) (131-0169) polyclonal antibody (1:150) overnight at 37°C. Cells were stained with Alexa Fluor® 488-conjugated goat anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue, 20X).

Storage/Stability

Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.

Expiration

12 months from the date of shipment when stored properly.