Anti-HACE1 Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Mouse
Accession Number	Q8IYU2
Gene Id	57531
Gene Symbols	HACE1
Protein Name / Synonyms	E3 ubiquitin-protein ligase HACE1, 6.3.2.-, HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1, HACE1, KIAA1320
Clonality	Monoclonal
Clone	1854CT757.66.47
Isotype	Kappa, IgG1
Recommended Applications	Western Blotting
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.

Other Information

Antigen Type

Recombinant Protein

Format

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Calculated Molecular Weight (Da)

102342

Antigen Source

Human

Images

WB (1:2000)

All lanes : Anti-HACE1 Antibody at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: 293T/17 whole cell lysate e Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 102 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products