Anti-HAGH Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q16775
Gene Id	3029
Gene Symbols	HAGH
Protein Name / Synonyms	HAGH, GLO2, GLX2, GLXII, HAGH1, hydroxyacylglutathione hydrolase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. Three transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100

Format

Calculated MW

25kDa/28kDa/33kDa

Observed MW

34kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human HAGH (NP_001035517.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HAGH antibody (144-06615) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - HAGH antibody (144-06615)

Immunohistochemistry of paraffin-embedded human liver cancer using HAGH antibody (144-06615) at dilution of 1:100 (40x lens).
Immunohistochemistry - HAGH antibody (144-06615)

Immunohistochemistry of paraffin-embedded human esophageal cancer using HAGH antibody (144-06615) at dilution of 1:100 (40x lens).
Immunohistochemistry - HAGH antibody (144-06615)

Immunofluorescence analysis of A549 cells using HAGH antibody (144-06615). Blue: DAPI for nuclear staining.
Immunofluorescence - HAGH antibody (144-06615)

Expiration:

12 months from the date of shipment when stored properly.

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