3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-HCK (N-term) Antibody
Catalog #: 102-17578
Rabbit Anti-Human HCK (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P08631 |
| Gene Id | 3055 |
| Gene Symbols | HCK |
| Protein Name / Synonyms | HCK (N-term) |
| Clonality | Polyclonal |
| Preparation | This HCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-156 amino acids from the N-terminal region of human HCK. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
HCK is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq].
Specificity
Predicted Reactivity
Mouse, Rat, Monkey
Other Names
HCK; Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck
NCBI Accession #
NP_001165600.1;NP_001165601.1;NP_001165602.1;NP_001165604.1;NP_002101.2
Other Accession #
P50545; P08103; Q95M30; NP_002101
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
59600
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | HCK Antibody (N-term) (Cat# 102-17578) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow). |
 | HCK Antibody (N-term) (Cat# 102-17578) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HCK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | HCK Antibody (N-term) (Cat# 102-17578) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.