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Anti-HDAC1 Antibody
Catalog #: 144-00238
HDAC1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13547 |
| Gene Id | 3065 |
| Gene Symbols | HDAC1 |
| Protein Name / Synonyms | HDAC1, GON-10, HD1, RPD3, RPD3L1, histone deacetylase 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:100 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
55kDa
Observed MW
62kDa
Immunogen
A synthetic peptide of human HDAC1
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HDAC1 antibody (144-00238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (144-00238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human lung cancer using HDAC1 antibody (144-00238) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using HDAC1 antibody (144-00238) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using HDAC1 antibody (144-00238) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HDAC1 antibody (144-00238) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using HDAC1 antibody (144-00238). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HT-29 cells, using 3 ug HDAC1 antibody (144-00238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (144-00238) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.