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Anti-HDAC3 Antibody
Catalog #: 144-02139
HDAC3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O15379 |
| Gene Id | 8841 |
| Gene Symbols | HDAC3 |
| Protein Name / Synonyms | HDAC3, HD3, RPD3, RPD3-2, histone deacetylase 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.05% proclin300, 50% glycerol, pH 7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP, ChIP |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
Format
Calculated MW
48kDa/49kDa
Observed MW
47kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 299-428 of human HDAC3 (NP_003874.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HDAC3 antibody (144-02139) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using HDAC3 Antibody (144-02139) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using HDAC3 Antibody (144-02139) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC3 Antibody (144-02139) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HDAC3 Antibody (144-02139) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF-7 cells using HDAC3 antibody (144-02139). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HDAC3 antibody (144-02139). Western blot was performed from the immunoprecipitate using HDAC3 antibody (144-02139) at a dilition of 1:1000.
Chromatin immunoprecipitation of extracts of 293T cell line, using HDAC3 antibody (144-02139) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Expiration:
12 months from the date of shipment when stored properly.