Anti-HDGFRP2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	84717
Gene Symbols	HDGFRP2
Protein Name / Synonyms	HDGFRP2, HDGF-2, HDGF2, HRP-2, HRP2, hepatoma-derived growth factor-related protein 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the hepatoma-derived growth factor (HDGF) family. The protein includes an N-terminal PWWP domain that binds to methyl-lysine-containing histones, with specific binding of this protein to tri-methylated lysines 36 and 79 of histone H3, and di- and tri-methylated lysine 20 of histone H4. The protein functions in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. Alternative splicing of this gene results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

74kDa

Observed MW

110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 501-670 of human HDGFRP2 (NP_116020.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse testis, using HDGFRP2 antibody (144-07484) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - HDGFRP2 antibody (144-07484)

Immunohistochemistry of paraffin-embedded mouse ileum using HDGFRP2 antibody (144-07484) at dilution of 1:100 (40x lens).
Immunohistochemistry - HDGFRP2 antibody (144-07484)

Immunofluorescence analysis of A549 cells using HDGFRP2 antibody (144-07484).
Immunofluorescence - HDGFRP2 antibody (144-07484)

Expiration:

12 months from the date of shipment when stored properly.

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