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Anti-Hepcidin Antibody
Catalog #: RB-13-0005
Rabbit Anti-Human Hepcidin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | P81172 |
| Gene Id | 57817 |
| Gene Symbols | HAMP HEPC LEAP1 UNQ487/PRO1003 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human Hepcidin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction will be purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | DTHFPICIFCCGCCHRSKCGMCCKT |
| Recommended Applications | ELISA (recommended work dilution= 1:10^6 (at least)), Western Blotting (recommended work dilution= 2-5 ug/mL) |
| Reconstitution | Supplied as lyophilized purified antibody. The antibody can be reconstituted in sterile water, be stored at 4°C for 1 month after resuspension. Please avoid freeze-thaw cycles. |
| Research Area | Cardiovascular Disease |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Hepcidin, also called as LEAP-1 is cysteine-rich peptide containing four disulfide bonds. Its active form, 25-residue peptide was identified in human blood. It was predominantly detected in the liver, and, to a much lower extent, in the heart. Gram-positive Bacillus megaterium, Bacillus subtilis, Micrococcus luteus, Staphylococcus carnosus, and Gram-negative Neisseria cinerea as well as the yeast Saccharomyces cerevisiae dose-dependently exhibited sensitivity upon treatment with synthetic Hepcidin. It is also the central regulator of systemic iron homeostasis. Dysregulation of hepcidin production results in a variety of iron disorders. Hepcidin deficiency is the cause of iron overload in hereditary hemochromatosis, iron-loading anemias, and hepatitis C. Hepcidin excess is associated with anemia of inflammation, chronic kidney disease and iron-refractory iron deficiency anemia.
Specificity
The antibody detects specifically synthetic immunogen. Cross reactivity with mouse and rat Hepcidin are not tested yet.
Storage
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
References
- Ganz T. Hepcidin, a key regulator of iron metabolism and mediator of anemia of inflammation". Blood (2003).
- Park CH, Valore EV, Waring AJ, Ganz T. Hepcidin, a urinary antimicrobial peptide synthesized in the liver. J. Biol. Chem (2001)
Expiration:
12 months from the date of shipment when stored properly.