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Anti-HEV71 Antibody
Catalog #: 130-10014
Rabbit Anti-HEV71 Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | AAX21195 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was a recombinant protein of human enterovirus 71-VP1 expressed in E.Coli. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000), Western Blotting (recommended work dilution= to be defined) |
| Antigen Catalog Number | 230-00013 |
| Reconstitution | Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Enterovirus 71 (EV-71) is notable as one of the major causative agents for hand, foot and mouth disease (HFMD), and is sometimes associated with severe central nervous system diseases. To date, little is known about the molecular mechanisms of host response to EV71 infection, but increases in the level of mRNAs encoding chemokines, proteins involved in protein degradation, complement proteins, and proapoptotis proteins have been implicated.
Specificity
The rabbit anti-enterovirus 71-VP1 antibody specifically detected target derived from human enterovirus 71-VP1 recombinant protein. The fine specificity to viral protein was not defined.
Storage
Store at -20°C, if not intended for use within a month, keep it at 4°C and minimize freezing and thawing when use.
References
- Mary Jane Cardosa,et al., (2003) Molecular Epidemiology of Human Enterovirus 71 Strains and Recent Outbreaks in the Asia-Pacific Region: Comparative Analysis of the VP1 and VP4 Genes. Emerg Infect Dis.9(4): 462468
- Kung SH, Wang SF, Huang CW, Hsu CC, Liu HF, Yang JY. Genetic and antigenic analyses of enterovirus 71 isolates in Taiwan during 1998-2005. Clin Microbiol Infect. 2007
Expiration:
12 months from the date of shipment when stored properly.