Anti-HEXIM1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	O94992
Gene Id	10614
Gene Symbols	HEXIM1
Protein Name / Synonyms	HEXIM1, CLP1, EDG1, HIS1, MAQ1, protein HEXIM1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

40kDa

Observed MW

70kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 210-359 of human HEXIM1 (NP_006451.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HEXIM1 antibody (144-05775) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - HEXIM1 antibody (144-05775)

Immunohistochemistry of paraffin-embedded rat brain using HEXIM1 Antibody (144-05775) at dilution of 1:100 (40x lens).
Immunohistochemistry - HEXIM1 Antibody (144-05775)

Immunohistochemistry of paraffin-embedded human colon cancer using HEXIM1 Antibody (144-05775) at dilution of 1:100 (40x lens).
Immunohistochemistry - HEXIM1 Antibody (144-05775)

Immunofluorescence analysis of A549 cells using HEXIM1 antibody (144-05775). Blue: DAPI for nuclear staining.
Immunofluorescence - HEXIM1 antibody (144-05775)

Expiration:

12 months from the date of shipment when stored properly.

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