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Anti-HIF-1-alpha (Pro564) Antibody
Catalog #: 130-11164
Rabbit Anti-Human HIF-1 alpha (HIF1A) Polyclonal Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg, 1 mg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q16665 |
| Gene Id | 3091 |
| Gene Symbols | HIF1A, BHLHE78, MOP1, PASD8 |
| Protein Name / Synonyms | Hypoxia-inducible factor 1-alpha (HIF-1-alpha, HIF1-alpha); ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78 (bHLHe78); Member of PAS protein 1; PAS domain-containing protein 8 |
| Clonality | Polyclonal |
| Preparation | This antibody was produced from antiserum of rabbits immunized with a synthetic immunogen HIF-1α (RLEMLAP[Hyp564]YIPMDKC). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified by Protein A affinity chromatography. |
| Immunogen | Synthetic peptide corresponding to the sequence RLEMLAPYIPMDKC from human HIF-1α, containing hydroxyproline at position Pro564. |
| Immunogen Sequence | RLEMLAP[Hyp564]YIPMDKC |
| Recommended Applications | ELISA (recommended work dilution = 1:40, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Angiogenesis, Cardiovascular Disease, Inflammation, HIF-1 alpha Signaling, Autophagy, Hypoxia Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Description
This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.