Anti-HIP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O00291
Gene Id	3092
Gene Symbols	HIP1
Protein Name / Synonyms	HIP1, HIP-I, ILWEQ, SHON, SHONbeta, SHONgamma, huntingtin-interacting protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The product of this gene is a membrane-associated protein that functions in clathrin-mediated endocytosis and protein trafficking within the cell. The encoded protein binds to the huntingtin protein in the brain; this interaction is lost in Huntington's disease. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

110kDa/113kDa/116kDa

Observed MW

120kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 410-690 of human HIP1 (NP_005329.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HIP1 antibody (144-06921) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - HIP1 Polyclonal Antibody (144-06921)

Immunohistochemistry of paraffin-embedded mouse brain using HIP1 antibody (144-06921) at dilution of 1:100 (40x lens).
Immunohistochemistry - HIP1 Polyclonal Antibody (144-06921)

Immunofluorescence analysis of MCF7 cells using HIP1 antibody (144-06921). Blue: DAPI for nuclear staining.
Immunofluorescence - HIP1 Polyclonal Antibody (144-06921)

Expiration:

12 months from the date of shipment when stored properly.

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