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Anti-HIST3H3 (Center) Antibody

Catalog #: 102-11457

Rabbit Anti-Human HIST3H3 (Center) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q16695
Gene Id
8290
Gene Symbols
HIST3H3
Protein Name / Synonyms
HIST3H3 (Center)
ClonalityPolyclonal
PreparationThis HIST3H3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids from the Central region of human HIST3H3.
Concentration (lot specific)
0.460 mg/ml
Recommended ApplicationsWestern Blotting, FC
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq].

Specificity

Other Names
HIST3H3; H3FT; Histone H3.1t; H3/g
NCBI Accession #
NP_003484.1
Other Accession #
NP_003484.1

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
15508
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
HIST3H3 Antibody (Center) (Cat# 102-11457) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST3H3 antibody detected the HIST3H3 protein (arrow).
HIST3H3 Antibody (Center) (Cat# 102-11457) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.