Anti-HK1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P19367
Gene Id	3098
Gene Symbols	HK1
Protein Name / Synonyms	HK1 (C-term)
Clonality	Polyclonal
Preparation	This HK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-734 amino acids from the C-terminal region of human HK1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.

Specificity

Other Names

HK1; Hexokinase-1; Brain form hexokinase; Hexokinase type I

NCBI Accession #

NP_000179.2;NP_277031.1;NP_277032.1;NP_277033.1;NP_277035.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

102486

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-HK1 Antibody (C-term) (Cat# 102-17823) in A375 cell line lysate. HK1(arrow) was detected using the purified Pab.
	Western blot analysis of HK1 (arrow) using HK1 Antibody (C-term) (Cat# 102-17823). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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