Anti-HK2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P52789
Gene Id	3099
Gene Symbols	HK2
Protein Name / Synonyms	HK2, HKII, HXK2, hexokinase-2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

102kDa

Observed MW

102kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HK2 (NP_000180.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HK2 antibody (144-00994) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - HK2 Polyclonal Antibody (144-00994)

Immunohistochemistry of paraffin-embedded rat heart using HK2 antibody (144-00994) at dilution of 1:200 (40x lens).
Immunohistochemistry - HK2 Polyclonal Antibody (144-00994)

Expiration:

12 months from the date of shipment when stored properly.

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