Anti-HK2 (Hexokinase II) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P52789
Gene Id	3099
Gene Symbols	HK2
Protein Name / Synonyms	HK2 (Hexokinase II) (N-term)
Clonality	Polyclonal
Preparation	This HK2 (Hexokinase II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-121 amino acids from the N-terminal region of human HK2 (Hexokinase II).
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Angiogenesis, Energy Metabolism, Glycolysis & Lipid Metabolism
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III, and IV. Hexokinase is an allosteric enzyme inhibited by its product GLC-6-P. Hexokinase activity is involved in the first step in several metabolic pathways. HK3 is bound to the outer mitochondrial membrane. Its hydrophobic N-terminal sequence may be involved in membrane bindng. It is the predominant hexokinase isozyme expressed in insuline-responsive tissues such as skeletal muscle. The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated wiht the N-terminus. Although found in NIDDM patients, genetic variations of HK2 do not contribute to the disease.

Specificity

Predicted Reactivity

Mouse, Rat, Pig

Other Names

HK2; Hexokinase-2; Hexokinase type II; Muscle form hexokinase

NCBI Accession #

NP_000180.2

Other Accession #

P27881; Q1W674; O08528

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

102380

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	HK2 Antibody (E106) (Cat# 102-17820) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the HK2 antibody detected the HK2 protein (arrow).
	Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with HK2 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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