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Anti-HK3 Antibody
Catalog #: 144-08428
HK3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P52790 |
| Gene Id | 3101 |
| Gene Symbols | HK3 |
| Protein Name / Synonyms | HK3, HKIII, HXK3, hexokinase-3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
99kDa
Observed MW
99kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HK3 (NP_002106.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HK3 antibody (144-08428) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse kidney using HK3 antibody (144-08428) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HK3 antibody (144-08428) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.