Anti-HLA-DPB1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04440
Gene Id	3115
Gene Symbols	HLA-DPB1
Protein Name / Synonyms	HLA-DPB1 (Center)
Clonality	Polyclonal
Preparation	This HLA-DPB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-105 amino acids from the Central region of human HLA-DPB1.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	FC, Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Specificity

Other Names

HLA-DPB1; HLA-DP1B; HLA class II histocompatibility antigen, DP beta 1 chain; HLA class II histocompatibility antigen, DP(W4) beta chain; MHC class II antigen DPB1

NCBI Accession #

NP_002112.3

Other Accession #

NP_002112.3

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

29159

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of HLA-DPB1 (arrow) using rabbit polyclonal HLA-DPB1 Antibody (Center) (Cat# 102-12186). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-DPB1 gene.
	HLA-DPB1 Antibody (Center) (Cat# 102-12186)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HLA-DPB1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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