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Anti-HLA-DRB4 (N-term) Antibody
Catalog #: 102-12100
Rabbit Anti-Human HLA-DRB4 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P13762 |
| Gene Id | 3126 |
| Gene Symbols | HLA-DRB4 |
| Protein Name / Synonyms | HLA-DRB4 (N-term) |
| Clonality | Polyclonal |
| Preparation | This HLA-DRB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-72 amino acids from the N-terminal region of human HLA-DRB4. |
| Concentration (lot specific) | 0.390 mg/ml |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
HLA-DRB4 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogues DRB3, DRB4 and DRB5. The presence of DRB4 is linked with allelic variants of DRB1, otherwise it is omitted. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9.
Specificity
Other Names
HLA-DRB4; HLA class II histocompatibility antigen, DR beta 4 chain; MHC class II antigen DRB4
NCBI Accession #
NP_068818.4
Other Accession #
NP_068818.4
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
29941
Recommended Dilutions
WB: 1:1000
 | Western blot analysis of lysate from Raji cell line, using HLA-DRB4 Antibody (N-term)(Cat# 102-12100). 102-12100 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.