RayBiotech

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ISO 13485:2016 cGMP

Anti-HLTF Antibody

Catalog #: 144-08353

HLTF Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
Q14527
Gene Id
6596
Gene Symbols
HLTF
Protein Name / Synonyms
HLTF, HIP116, HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3, ZBU1, helicase-like transcription factor
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
99kDa/113kDa
Observed MW
114kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 726-1009 of human HLTF (NP_620636.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HLTF antibody (144-08353) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - HLTF Polyclonal Antibody (144-08353)

Expiration:
12 months from the date of shipment when stored properly.