Anti-HMGA1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P17096
Gene Id	3159
Gene Symbols	HMGA1
Protein Name / Synonyms	HMGA1 (C-term)
Clonality	Polyclonal
Preparation	This HMGA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 64-93 amino acids from the C-terminal region of human HMGA1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HMGA1 encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus.

Specificity

Predicted Reactivity

Mouse, Rat, Hamster

Other Names

HMGA1; HMGIY; High mobility group protein HMG-I/HMG-Y; High mobility group AT-hook protein 1; High mobility group protein R

NCBI Accession #

NP_002122.1;NP_665906.1;NP_665908.1;NP_665909.1;NP_665910.1;NP_665912.1

Other Accession #

Q8K585; P17095; Q9QXP3

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

11676

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; FC: 1:10-50

	Fluorescent image of A549 cell stained with HMGA1 Antibody (C-term)(Cat# 102-18144).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HMGA1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).HMGA1 immunoreactivity is localized to Nucleus significantly.
	HMGA1 Antibody (C-term) (Cat# 102-18144) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HMGA1 antibody detected the HMGA1 protein (arrow).
	HMGA1 Antibody (C-term) (Cat# 102-18144) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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