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Anti-HMGB1 Antibody
Catalog #: 144-02553
HMGB1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P09429 |
| Gene Id | 3146 |
| Gene Symbols | HMGB1 |
| Protein Name / Synonyms | HMGB1, HMG-1, HMG1, HMG3, SBP-1, high mobility group box 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Angiogenesis, Cancer, Inflammation, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
24kDa
Observed MW
29kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HMGB1 (NP_002119.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HMGB1 antibody (144-02553) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot analysis of extracts from normal (control) and HMGB1 knockout (KO) 293T cells, using HMGB1 antibody (144-02553) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human amygdalitis using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human adenomyosis using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using HMGB1 Antibody (144-02553) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HMGB1 antibody (144-02553) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.