Anti-HMGCR Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P04035
Gene Id	3156
Gene Symbols	HMGCR
Protein Name / Synonyms	HMGCR, LDLCQ3, 3-hydroxy-3-methylglutaryl-CoA reductase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:1000 - 1:4000

Format

Calculated MW

92kDa/97kDa/99kDa

Observed MW

110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 629-888 of human HMGCR (NP_000850.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HMGCR antibody (144-01633) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - HMGCR antibody (144-01633)

Expiration:

12 months from the date of shipment when stored properly.

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