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Anti-HMGN1 Antibody
Catalog #: 144-06155
HMGN1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P05114 |
| Gene Id | 3150 |
| Gene Symbols | HMGN1 |
| Protein Name / Synonyms | HMGN1, HMG14, non-histone chromosomal protein HMG-14 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
10kDa
Observed MW
17kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGN1 (NP_004956.5).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HMGN1 antibody (144-06155) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using HMGN1 antibody (144-06155) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using HMGN1 antibody (144-06155) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.