RayBiotech

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ISO 13485:2016 cGMP

Anti-HMOX2 Antibody

Catalog #: 144-02745

HMOX2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P30519
Gene Id
3163
Gene Symbols
HMOX2
Protein Name / Synonyms
HMOX2, HO-2, heme oxygenase 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:1000

Format

Calculated MW
32kDa/36kDa
Observed MW
37kDa

Immunogen

Recombinant protein of human HMOX2

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HMOX2 antibody (144-02745) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - HMOX2 antibody (144-02745)

Expiration:
12 months from the date of shipment when stored properly.