3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-HNMT Antibody
Catalog #: 144-60362
HNMT Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P50135 |
| Gene Id | 3176 |
| Gene Symbols | HNMT |
| Protein Name / Synonyms | HNMT, HMT, HNMT-S1, HNMT-S2, MRT51, histamine N-methyltransferase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:20 - 1:50
IHC 1:50 - 1:200
IP 1:20 - 1:50
Format
Calculated MW
6kDa/14kDa/33kDa
Observed MW
27kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HNMT antibody (144-60362) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using HNMT antibody (144-60362) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 1ug HNMT antibody (144-60362). Western blot was performed from the immunoprecipitate using HNMT antibody (144-60362) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.