HNRNPA0 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13151 |
| Gene Id | 10949 |
| Gene Symbols | HNRNPA0 |
| Protein Name / Synonyms | HNRNPA0, HNRPA0, heterogeneous nuclear ribonucleoprotein A0 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene belongs to the A/B subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind RNAs, followed by a glycine-rich C-terminus.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IP 1:50 - 1:100
IHC 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
30kDa
Observed MW
37kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HNRNPA0 (NP_006796.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HNRNPA0 antibody (144-06029) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using HNRNPA0 antibody (144-06029) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug HNRNPA0 antibody (144-06029). Western blot was performed from the immunoprecipitate using HNRNPA0 antibody (144-06029) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.
